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Leisure time is an important aspect in students` life which is closely related with development of students’ personality based on personal interests and preferences. As a content of leisure time, sport activities could be a very useful tool to improve student’s health and well-being and to create a habits for healthy and active lifestyle. Considering the importance of participation in sport activities in leisure time, we conducted a research that examine students interests and participation in sport activities during leisure time across three different countries as well as to determine the differences in national awareness raising importance of sport for healthy lifestyle among university students. The research was realized on a sample of 354 respondents, students at Teaching Faculties in Macedonia, Slovenia and Bulgaria. The research was realized using specially designed questionnaire, composed of 12 questions. Obtained results were analyzed using descriptive statistics parameters, while differences among students across three countries were determined using analyses of variance (ANOVA). Based on obtained result, we noted that the “lack of time” spended in sport activities and decress of participation of sport during studies are common for all three countries. Differences between countries are determined in most of the questions related to the time spend in participation in sport activities, motivation for sport, oppinions of the importance of sport in everyday life, preferences for different sport activities etc. Obtained results arise the question for importance of the universitiy educaton in raising the awareness for importance of sport for health and lifelong well-being.